pH-responsive, reactive oxygen species scavenging and highly swellable nanogel for colon-targeted oral drug delivery

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dc.contributor.author Kumawat, Akshant
dc.contributor.author Karmakar, Mrinmoy
dc.contributor.author Ghoroi, Chinmay
dc.coverage.spatial United States of America
dc.date.accessioned 2024-08-09T10:31:54Z
dc.date.available 2024-08-09T10:31:54Z
dc.date.issued 2024-08
dc.identifier.citation Kumawat, Akshant; Karmakar, Mrinmoy and Ghoroi, Chinmay, "pH-responsive, reactive oxygen species scavenging and highly swellable nanogel for colon-targeted oral drug delivery", ACS Applied Nano Materials, DOI: 10.1021/acsanm.4c02769, Aug. 2024.
dc.identifier.issn 2574-0970
dc.identifier.uri https://doi.org/10.1021/acsanm.4c02769
dc.identifier.uri https://repository.iitgn.ac.in/handle/123456789/10305
dc.description.abstract In the realm of colon-based drug delivery, developing a pH-responsive nanocarrier that exhibits significant intrinsic reactive oxygen species (ROS) scavenging activity holds great promise. To address this, a nanogel (NG) is synthesized using itaconic acid (IAc) and acrylamide (AAm) monomers in a molar ratio of 1:4 via free radical polymerization. The spherical NG of size 190 ± 15 nm is confirmed by using field emission scanning electron microscopy (FESEM). Dynamic light scattering (DLS) characterization reveals a hydrodynamic diameter and zeta potential of 271 ± 23 nm and −6.9 ± 2.3 mV, respectively. FTIR, XPS, and NMR analyses confirm the presence of multiple functionalities on the NG. Significant improvement in swelling (10 times) at colonic pH (pH 7.4) in contrast to gastric pH (pH 1.2) ensures the pH-responsive behavior of a NG along with five times higher ROS scavenging activity compared to control. As a model drug, doxorubicin (DOX) is employed to investigate release properties and cellular uptake. The NG exhibited drug loading capacity and efficiency of 26 ± 1.2% and 90 ± 1.3%, respectively, with a three times higher DOX release at pH 7.4 than at pH 1.2. Rheology data reveal the superior structural integrity of the doxorubicin loaded nanogel (DNG) compared to the NG. The biocompatibility of the NG is confirmed through MTT, the hemolysis assay, and cell uptake assays on HCT-116 colon cancer cells. The cellular uptake studies have indicated NG-mediated drug release in the colon microenvironment with subsequent passive diffusion of DOX into cells. These findings underscore the capability of the synthesized NG as a vehicle for oral drug delivery to the colon.
dc.description.statementofresponsibility by Akshant Kumawat, Mrinmoy Karmakar and Chinmay Ghoroi
dc.language.iso en_US
dc.publisher American Chemical Society
dc.subject Nanogels
dc.subject Poly[itaconic acid-co-acrylamide]
dc.subject Oral route of administration
dc.subject Colon drug delivery
dc.subject pH-responsive system
dc.subject Swelling properties
dc.subject ROS/RNS scavenging
dc.title pH-responsive, reactive oxygen species scavenging and highly swellable nanogel for colon-targeted oral drug delivery
dc.type Article
dc.relation.journal ACS Applied Nano Materials


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