Abstract:
The 'power house' of the cell mitochondria upsurges significant interest in its investigation of imaging and mapping of the physiological processes. By utilizing sterol-modified rhodamine, we have performed live-cell imaging of mitochondria without dependence on membrane potential. The sterol probes are highly biocompatible, and can track the mitochondrial live-cell dynamics in a background free manner with improved brightness and impressive contrast. This is a first attempt to study the stress response using a direct fluorescence readout by bio-conjugates of rhodamine inside mitochondria. The results pave the way for developing different sterol markers for understanding the cellular response and function